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  |   1 INSTITUTO TECNOLÓGICO DE MORELIA DIVISIÓN DE ESTUDIOS DE LICENCIATURA BIOQUÍMICA I ING. BIOQUÍMICA PRÁCTICA 1 TEMA: INDICADORES DE Ph Y BUFFERS  ALUMNOS: JUAN JOSÉ DÍAZ AGUILARARTURO DÍAZ PROFESOR: D.C. JUAN CARLOS GONZÁLEZ HERNÁNDEZ   MORELIA, MICHOACÁN, MÉXICO; FEBRERO 2! 2  INTRODUCCION L# $%&'()& * + +$+'- /%+ -(+&+& '(+0-1 '3%+1-1 140+ 1 1+0+1 5(51 +1-6&#3(#+&-+ 0+#'(&#71 '& 1%1 303(+7#7+1 /%+ 1 8#'+& 6'(71  #'#(&1 *3#0# 7+-+0(&#0 1% #'(7+9  #'#(&(7#7 1+ 8#'+ %1 %& +7(70 7+ 3H. A&-+1 7++&-0#0 7+ +& # 8#4#0 7+ 1 +7(1 %-((9#71 3#0# '&'+0 + 0#7 7+ #'#(&(7#7 #'(7+9 +& %& '3%+1- +1 (30-#&-+ /%+ +3(/%+1 /%< +1 + 3H * '%6+1 1&#1 '#0#'-+0=1-('#1 /%+ 7(1-(&%+& # %& 6'(7 7+ %&# 4#1+  1%1-#&'(# #'#(&#.E 3H +1 # $0# +& /%+ 1+ +30+1# # #'(7+9  #'#(&(7#7 7+ %&# 1%1-#&'(# # 3#0-(0 7+ 1% '&'+&-0#'()& #0 7+ 30-&+1  3-+&'(# 7+ 8(70)+&. E1- /%(+0+ 7+'(0 /%+ 1+ %-((9# ' %&# %&(7#7 7+ +7(7# 3#0# +30+1#0 # '&'+&-0#'()& 7+8(70&+1 +& %&# 1%1-#&'(#. A 3#0-(0 7+ +1-%7( 7+ '30-#(+&- 7+ #%# '6'(7 * 4#1+ 1+ '0+) %&# +1'## /%+ 5# 7+17+  8#1-# !>, '00+13&7(+&7 15#0+1 +&0+1 7+ ? # # #'(7+9 * 1 #*0+1 7+ ? # # #'#(&(7#7 7+ # 1%1-#&'(#+& '%+1-()&, 1(+&7 +& +1-+ '#1 + ? %& 5#0 /%+ '00+13&7+ # %& 3H &+%-0.M%'8#1 7+ #1 0+#''(&+1 /%=('#1 /%+ 1+ 307%'+& +& 1%'()& #'%1# &+'+1(-#&/%+ + 3H 7+ 1(1-+# 1+ #&-+&# '&1-#&-+, 3#0# +5(-#0 /%+ '%00#& -0#10+#''(&+1 & 7+1+#7#1.L#1 1%'(&+1 0+%#70#1  @4%$$+0 1& '#3#'+1 7+ #&-+&+0 # #'(7+9  4#1('(7#77+ %& 1(1-+# 7+&-0 7+ %& (&-+05# 0+7%'(7 7+ 3H, 30  '%# -(+&+& -(3+1#3('#'(&+1, -#&- +& # (&7%1-0(# ' +& 1 #40#-0(1.E1-#1 1%'(&+1 '&-(+&+& ' +13+'(+1 30+7(&#&-+1, %& 3#0 6'(7  4#1+'&%#7 +& '&'+&-0#'(&+1 #30+'(#4+1. M#*0+1 /%+ !  2 M S+ 3%+7+30+3#0#0 7(15(+&7 +& #%# '#&-(7#7+1 #7+'%#7#1 7+ %& 6'(7 7<4( * %&# 1# 7+1% 4#1+ '&%#7#,  %&# 4#1+ 7<4( * %&# 1# 7+ 1% 6'(7 '&%#7; -#4(<& 1+3%+7+ 4-+&+0 %&# 1%'()& 0+%#70# 8#'(+&7 0+#''(&#0 3#0'(#+&-+ 30 &+%-0#(9#'()& %& 6'(7 7<4( '& %&# 4#1+ $%+0-+,  %&# 4#1+ 7<4( '& %& 6'(7$%+0-+.U&# 5+9 $0#7# # 1%'()& 0+%#70#, + 3H 5#0=# 3' 30 + #0+#7 7+3+/%+#1 '#&-(7#7+1 7+ %& 6'(7 $%+0-+  7+ %&# 4#1+ $%+0-+, * 3(+07+ 1% '#3#'(7#70+%#70# 30 + #0+#7 7+ #%# 7(%'()&.L# '#3#'(7#7 #0-(%#70# 7+ %&# 1%'()& 1+ 7+$(&+ ' + &+0 7+ +1 7+%& 6'(7 $%+0-+  7+ %&# 4#1+ $%+0-+ /%+ '#1(&# %& '#4( 7+ %&# %&(7#7 +& + 3H+& ! (-0 7+ 1%'()& #0-(%#70#. L# '#3#'(7#7 7+ %& #0-(%#70 7+3+&7+ 7+ #'&'+&-0#'()& --# 7+ 1%1 '3&+&-+1 * 7+ # 0+#'()& 7+ '&'+&-0#'(&+1. L#'#3#'(7#7 7(1(&%*+ '& 0#3(7+9 # +7(7# /%+ # 0+#'()& 7+ 6'(7 '& 0+13+'- ## 4#1+ '&%#7# 1+ #+# 7+ # %&(7#7.E '30-#(+&- 7+ %& 4%$$+0 1+ 3%+7+ 1+%(0 '& # +'%#'()& 7+ H+&7+01&H#11+4#'8, # '%# &1 3+0(-+ '#'%#0 + 3H 7+ %&# 1%'()& #0-(%#70#3H J 3K#  B#1+ '&%#7#A'(7 7<4( 3  OBJETIVOS  P0+3#0#0 1%1-#&'(#1 #0-(%#70#1 '& 7($+0+&-+1 '&'+&-0#'(&+1 7+1%1-#&'(#1  V+0($('#0 1 3H 7+ '#7# 1%1-#&'(# 30+3#0#7#   A30+&7+0 # %-((9#0 + 3-+&'()+-0 3#0# 37+0 +7(0 1 3H 7+ '#7#1%1-#&'(#. 4
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